Molecular detection of polycystic kidney disease in Persian and Persian-related breeds in Iran

Abstract

Objectives: This study was aimed at detecting feline autosomal dominant polycystic kidney disease in a population of Persian and Persian-related breeds by a molecular method in Iran. Methods: Buccal swab samples were collected from 47 cats and examined with a touchdown PCR method. Additionally, partial sequencing was performed in two cats with bilateral renal cysts. Results: Twenty-two cats (46.8%) were diagnosed as heterozygous for this mutation. Sequence analysis of two cats showed C to A point mutation in the PKD1 gene, as in previous studies. Conclusions and relevance: Prevalence of this disease is high in Iran, highlighting the need for molecular screening tests before including cats in breeding programmes.