Total antigen from Leishmania (Leishmania) amazonensis and isolates from the Leishmania braziliensis complex, along with their respective antigenic fractions obtained by affinity chromatography on concanavalin-A-Sepharose and jacalin-agarose columns evaluated using immunoenzymatic ELISA assay. For this, serum samples from 229 patients were used, grouped as American tegmental leishmaniasis (no=58), visceral leishmaniasis (no=28), Chagas disease (no=49), malaria (no=32), tuberculosis (no=13) and healthy volunteers (no=49). Samples from American tegmentary leishmaniasis showed higher reactivity with antigens isolated from the Leishmania braziliensis complex than with antigens from Leishmania amazonensis (p<0.001). ELISA assays showed a sensitivity range from 60% to 95% with antigens isolated from the Leishmania braziliensis complex. There was marked nonspecific reactivity among serum samples with the use of antigenic fractions binding with concanavalin-A and jacalin from both Leishmania complexes, in comparison with other antigens (p<0.001). The results presented in this study suggest that the use of homologous antigens increases the efficiency of anti-Leishmania immunoglobulin detection, which may be very valuable for diagnostic purposes.
CITATION STYLE
Gomes-Silva, A., Souza, M. A., Afonso-Cardoso, S. R., Andrade, L. R., Dietze, R., Lemos, E., … Ferreira, M. S. (2008). Serological reactivity of different antigenic preparations of Leishmania (Leishmania) amazonensis and the Leishmania braziliensis complex. Revista Da Sociedade Brasileira de Medicina Tropical, 41(2), 135–141. https://doi.org/10.1590/S0037-86822008000200001
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