Abstract
Transcription is a process by which the rate of RNA synthesis is regulated. Here we describe the techniques for carrying out promoter-reporter assays, electrophoretic mobility shift assays, chromosome conformation capture (3C) assays, chromatin immunoprecipitation assays, and CRISPR-Cas9 assay, five commonly used methods for studying and altering gene transcription.
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Jin, H., Sophocleous, A., Azfer, A., & Ralston, S. H. (2019). Analysis of transcriptional regulation in bone cells. In Methods in Molecular Biology (Vol. 1914, pp. 145–167). Humana Press Inc. https://doi.org/10.1007/978-1-4939-8997-3_8
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