Chitosan cushioned air stable single PEGylated phospholipid bilayers

Abstract

Air stable single lipid bilayers formation through SUVs fusion methods is still a challenge work. Here soluble LMW chitosan chemically coupled to silicon dioxide surface is cushioned for PEGylated lipid bilayers. Using imaging ellipsometry and spectroscopic ellipsometer, the influence of molar fraction of DSPE-PEG2000 for lipid bilayers formation and stability is examined. Under the mushroom phase, PEGylated lipid vesicles may aggregate to multiple bilayers; while entering the brush regime of PEG, vesicles can fused into single lipid bilayers. It is demonstrated both the appropriate mole fraction of PEGylaed lipid and hydrophilic polymer cushion are equally critical for achieving air and wash stable single lipid bilayers. Imaging ellipsometry also show the promise as an easy and useful tool for future air stable lipid bilayers based biosensor. © 2009 Springer-Verlag.