Deletion of activating Fcγ receptors does not confer protection in murine cryoglobulinemia-associated membranoproliferative glomerulonephritis

Abstract

Many types of glomerulonephritis are initiated by the deposition of immune complexes, which induce tissue injury via either engagement of Fc receptors on effector cells or via complement activation. Four murine Fcγ receptors (FcγRs) have been identified at present. Ligand binding to FcγRI, III , and IV induces cell activation via the immunoreceptor tyrosine-based activation motif on the common γ chain (FcRγ). In this study, FcRγchain knockout (FcRγ-/-) mice were crossed with thymic stromal lymphopoietin transgenic (TSLPtg) mice, which develop cryoglobulinemic membranoproliferative glomerulonephritis (MPGN). Female mice were studied at 30 and 50 days of age, when MPGN is in early and fully developed stages, respectively. Both TSLPtg and TSLPtg/FcRγ-/- mice developed MPGN with massive glomerular immune deposits, mesangial cell proliferation, extensive mesangial matrix accumulation, and macrophage influx. TSLPtg/FcRγ-/- mice had more glomerular immune complex deposits and higher levels of circulating cryoglobulins, IgG2a, IgG2b, and IgM, compared with TSLPtg mice. TSLPtg and TSLPtg/FcRγ-/- mice developed similar levels of proteinuria. These results demonstrated that deletion of activating FcγRs does not confer protection in this model of immune complexmediated MPGN. The findings contradict accepted paradigms on the role of activating FcγRs in promoting features of glomerulonephritis as seen in other model systems. We speculate engagement of FcγRs on cells such as monocytes/macrophages may be important for the clearance of deposited immune complexes and extracellular matrix proteins. Copyright © American Society for Investigative Pathology.