Role and mechanism of circ-PRKCI in hepatocellular carcinoma

26Citations
Citations of this article
15Readers
Mendeley users who have this article in their library.
Get full text

Abstract

BACKGROUND The circular RNA circ-PRKCI is an endogenous non-coding RNA that forms a covalently closed ring after reverse splicing, which plays a key role in the occurrence and development of multiple digestive system tumors. AIM To investigate the role and mechanism of circ-PRKCI in the occurrence and development of hepatocellular carcinoma (HCC). METHODS This study used real-time polymerase chain reaction to detect the expression of circ-PRKCI in tumor tissues, tumor adjacent tissues, and blood in patients with HCC and other digestive system tumor cells. A series of functional tests were performed to explore whether circ-PRKCI affects the growth of HCC cells and what is its mechanism in HCC. Meanwhile, fluorescence in situ hybridization was used to detect the subcellular localization of circ-PRKCI. Survival analysis was performed to predict the correlation between circ-PRKCI and the prognosis of HCC. Chi-square test and t-test were performed for statistical analyses. RESULTS The level of circ-PRKCI was significantly higher in HCC tissues than in tumor adjacent tissues, and in HCC cell lines than in cells lines of esophageal, liver, stomach, and colon cancers. A series of functional tests showed that circ-PRKCI substantially inhibited cell apoptosis and promoted cell invasion. It was found that circ-PRKCI can act as the sponge of miRNA-545 to reduce the expression of AKT3 protein. Moreover, the result of survival analysis showed that circ-PRKCI target gene E2F7 can reduce liver cancer patients' survival rate. And clinical data suggested that the distribution of circ-PRKCI rose with the depth of invasion, lymph node metastasis, distant metastasis, and TNM stage, indicating that circPRKCI may affect the survival and prognosis of patients with HCC by regulating E2E7. CONCLUSION This study explores the role and mechanism of circ-PRKCI in HCC, which provides a new research direction and theoretical basis for the treatment of HCC.

References Powered by Scopus

Circular RNAs are the predominant transcript isoform from hundreds of human genes in diverse cell types

2097Citations
N/AReaders
Get full text

The biogenesis and emerging roles of circular RNAs

1375Citations
N/AReaders
Get full text

Noncoding effects of circular RNA CCDC66 promote colon cancer growth and metastasis

555Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Epigenetic associations between lncRNA/circRNA and miRNA in hepatocellular carcinoma

129Citations
N/AReaders
Get full text

LncRNA MYLK-AS1 facilitates tumor progression and angiogenesis by targeting miR-424-5p/E2F7 axis and activating VEGFR-2 signaling pathway in hepatocellular carcinoma

93Citations
N/AReaders
Get full text

Circular RNAs in hepatocellular carcinoma: Biomarkers, functions and mechanisms

82Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Qi, S. X., Sun, H., Liu, H., Yu, J., Jiang, Z. Y., & Yan, P. (2019). Role and mechanism of circ-PRKCI in hepatocellular carcinoma. World Journal of Gastroenterology, 25(16), 1964–1974. https://doi.org/10.3748/wjg.v25.i16.1964

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 6

55%

Researcher 3

27%

Professor / Associate Prof. 1

9%

Lecturer / Post doc 1

9%

Readers' Discipline

Tooltip

Medicine and Dentistry 6

55%

Biochemistry, Genetics and Molecular Bi... 2

18%

Immunology and Microbiology 2

18%

Computer Science 1

9%

Save time finding and organizing research with Mendeley

Sign up for free