PENGARUH SENYAWA DELIGNIFIKASI DAN HIDROLISIS ASAM DENGAN PENAMBAHAN FeSO4 PADA PRODUKSI GLUKOSA DARI SPIRODELA POLYRHIZA

Abstract

Spirodela polyrhiza is a free-floating, fast-growing, and widely distributed aquatic plant in calm or slow-flowing bodies of water. The hydrolysis process in Spirodela polyrhiza was hampered due to the presence of lignin content. This study aims to study the effect of adding base compounds on the delignification process and the effect of FeSO4 concentration on the hydrolysis process on glucose production from Spirodela polyrhiza. The glucose production process is carried out through several stages, namely the process of making Spirodela polyrhiza flour, the chemical pretreatment process (delignification), and the acid hydrolysis process. The delignification and hydrolysis processes were carried out in an oven at a temperature of 121°C and a pressure of 1 atm. The delignification compounds used in the delignification process are NaOH, KOH, and Ca(OH)2. In the hydrolysis process, FeSO4 compounds were added at various concentrations, namely 20 mmol/L, 40 mmol/L, and 80 mmol/L. The results showed that the lowest glucose level was 4.55% which was obtained in the delignification treatment using Ca(OH)2 compound and the addition of a FeSO4 catalyst of 20 mmol/L. Meanwhile, the highest glucose level was 5.79% which was obtained by delignification treatment using NaOH compound and the addition of 80 mmol/L FeSO 4 catalyst in the acid hydrolysis process. NaOH is the most effective compound in the delignification process and the addition of FeSO4 in the hydrolysis process can increase levels of glucose obtained.