RNA metabolic labeling is a method of choice in the study of dynamic changes in the rate of gene transcription and RNA processing. It is particularly applicable to transcription of the ribosomal RNA genes and their processing products due to the very high levels of ribosomal RNA synthesis. Metabolic labeling can detect changes in ribosomal RNA transcription that occur within a few minutes as opposed to the still widely used RT-PCR or Northern blot procedures that measure RNA pool sizes and at best are able to detect changes occurring over several hours or several days. Here, we describe a metabolic labeling technique applicable to the measurement of ribosomal RNA synthesis and processing rates, as well as to the determination of RNA Polymerase I transcription elongation rates.
CITATION STYLE
Stefanovsky, V. Y., & Moss, T. (2016). Metabolic labeling in the study of mammalian ribosomal RNA synthesis. In Methods in Molecular Biology (Vol. 1455, pp. 133–145). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3792-9_11
Mendeley helps you to discover research relevant for your work.