Isolation of proteins from polyacrylamide gels

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Abstract

Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here, we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals. © 2012 Springer Science+Business Media, LLC.

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Michalk, I., Koristka, S., Arndt, C., & Bachmann, M. (2012). Isolation of proteins from polyacrylamide gels. Methods in Molecular Biology, 869, 427–431. https://doi.org/10.1007/978-1-61779-821-4_35

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