Protein-coated microcrystals (PCMC) are a high-activity preparation of enzymes for use in low-water media. The protocols for the preparation of PCMCs of Subtilisin Carlsberg and Candida antarctica lipase B (CAL B) are described. The combi-PCMC concept is useful both for cascade and non-cascade reactions. It can also be beneficial to combine two different specificities of a lipase when the substrate requires it. Combi-PCMC of CALB and Palatase used for the conversion of coffee oil present in spent coffee grounds to biodiesel is described. Cross-linked protein-coated microcrystals (CL-PCMC) in some cases can give better results than PCMC. Protocols for the CLPCMC of Subtilisin Carlsberg and Candida antarctica lipase B (CAL B) are described. A discussion of their applications is also provided.
CITATION STYLE
Mukherjee, J., & Gupta, M. N. (2017). Protein-coated microcrystals, combi-protein-coated microcrystals, and cross-linked protein-coated microcrystals of enzymes for use in low-water media. In Methods in Molecular Biology (Vol. 1504, pp. 125–137). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6499-4_10
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