Producción Fúngica de Proteasas Inducidas con Pelo de Cerdo

Abstract

The production of new proteases induced with pig hair was evaluated using two systems, submerged culture and solid state culture. Culture medium was Czapek-Dox for both systems. Enzyme production kinetic was monitored each 24 hours during 120 hours. Protelytic activity was evaluated with the azocasein method and biomass production was determined by the gravimetric method. Rhizopus oryzae was the microorganism with the capacity of using the pig hair as the single carbonnitrogen source, producing in submerged fermentation the highest enzymatic titers. The effect of pH, the substrate pretreatment and addition of different levels of substrate on proteolyitic activity and biomass production were determined. Results permitted to obtain titers of proteolytic activity of 975 U/L.