Affinity processing of cell-containing feeds using monolithic macroporous hydrogels, cryogels

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Abstract

Monolithic macroporous hydrogels, "cryogels," are produced by polymerization in a partially frozen state when the ice crystals perform as a porogen. Cryogels have a unique combination of properties: (i) large (10-100 μm) pores; (ii) minimal non-specific interactions due to the hydrophilic nature of the polymers; (iii) porosities exceeding 80-90%; (iv) good mechanical stability. These properties of cryogels allow for their application for direct capture of extracellularly expressed histidine-tagged protein from the fermentation broth and separation of different cell types. © Humana Press.

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Galaev, I. Y., & Mattiasson, B. (2007). Affinity processing of cell-containing feeds using monolithic macroporous hydrogels, cryogels. Methods in Molecular Biology, 421, 247–255. https://doi.org/10.1007/978-1-59745-582-4_16

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