Probing mitosis by manipulating the interactions of mitotic regulator proteins using rapamycin-inducible dimerization

Abstract

Inducible dimerization is a general approach to experimentally manipulate protein–protein interactions with temporal control. This chapter describes the use of rapamycin-inducible dimerization to manipulate mitotic regulatory proteins, for example to control kinetochore localization. A significant feature of this method relative to previously described protocols is the depletion of endogenous FKBP12 protein, which markedly improves dimerization efficiency.