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Quantitative Analysis of Reducing Sugars by 3, 5-Dinitrosalicylic Acid (DNSA Method)

  • Jain A
  • Jain R
  • Jain S
DOI: 10.1007/978-1-4939-9861-6_43
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Abstract

The method is based on the detection of presence of free carbonyl C=O group of reducing sugars. Initially oxidation of the ketonic and aldehyde functional group of fructose and glucose, respectively, by 3, 5-Dinitrosalicylic acid (yellow color) to by 3-amino-5-nitrosalicylic acid (orange-red)∗ in alkaline medium.

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Jain, A., Jain, R., & Jain, S. (2020). Quantitative Analysis of Reducing Sugars by 3, 5-Dinitrosalicylic Acid (DNSA Method) (pp. 181–183). https://doi.org/10.1007/978-1-4939-9861-6_43

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