Determination of phenolic content and antioxidant capacity of Launaea resedifolia from Algerian Sahara

Abstract

The present inquiry attempts to determine the antioxidant capacity of Launaea resedifolia’s hydroalcoholic extract which is obtained from Algerian Sahara. The antioxidant capacities of various extracts of aerial parts of L. resedifolia were estimated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging capacity, ferric reducing power (FRP), and total antioxidant capacity. In addition, the total phenols, flavonoids, and tannins contents of various extracts were measured by using colorimetric methods, the greater content of phenols and flavonoids was registered in crude extract (6.642 ± 0.262 mg gallic acid equivalent/g DW and 0.929 ± 0.018 mg quercetin equivalent/g DW, respectively), while the tannins was found in water fraction (1.246 ± 0.153 mg RE/g DW). Both capacities of DPPH scavenging and FRP were found best in ethyl acetate fraction (IC50 = 403.551 µg/ml and AEAC = 0.151 mM, respectively). For total antioxidant capacity, the superior capacity was observed in water fraction (EEAC = 0.149 Mm).