Reciprocal Subtraction Differential RNA Display (RSDD)

Abstract

Identification of differentially expressed genes is an essential step in comprehending the molecular basis of complex physiological\rand pathological processes. Subtraction hybridization and differential RNA display (DDRT-PCR) are two methods that are widely\rand successfully employed to clone differentially expressed genes. Unfortunately, both methods have inherent problems and\rlimitations requiring improvements in the technique. A combination of these two methods termed reciprocal subtraction differential\rRNA display is described here that considerably reduces the complexity of DDRT-PCR and facilitates the rapid and efficient\ridentification and cloning of both abundant and rare differentially expressed genes.