Dry preservation has become an attractive approach for the long-term storage of biologics. By removing water from the matrix to solidify the sample, refrigeration needs are reduced, and thus storage costs are minimized and shipping logistics greatly simplified. This chapter describes two energy deposition technologies, namely, microwave and laser systems, that have recently been used to enhance the rate and nature of solution densification for the purpose of anhydrous preservation of feline oocytes, sperm, and egg white lysozyme in trehalose glass. Several physical screening methodologies used to determine the suitability of an amorphous matrix for biopreservation are also introduced in this chapter.
CITATION STYLE
Wang, S., Trammell, S., & Elliott, G. D. (2021). Microwave- and laser-assisted drying for the anhydrous preservation of biologics. In Methods in Molecular Biology (Vol. 2180, pp. 203–220). Humana Press Inc. https://doi.org/10.1007/978-1-0716-0783-1_7
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