Heterogeneous post-translational modification of Actinobacillus actinomycetemcomitans fimbrillin

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Abstract

Fresh isolates of Actinobacillus actinomycetemcomitans produce bundle-forming fimbriae. The exact molecular mass of A. actinomycetemcomitans fimbrillin, a structural subunit of fimbriae, was determined by liquid chromatography-electrospray ionization mass spectrometry. Three major molecular species with 6,226.0, 6,366.0, and 6,513.0 Da were detected in a purified fimbrial fraction from the strain 310-a. These molecular masses were significantly higher than the molecular weight (5,118 Da) calculated from nucleotide sequence data of the fimbrillin gene, flp, suggesting that the fimbrial peptides were post-translationally modified. Modification of the fimbrial peptides was also suggested by an N-terminal amino acid sequence analysis of fimbrillin peptic fragments, with the modified amino acids being due to seven serine or asparagine residues located in the C-terminal region. A periodate oxidation/biotin-hydrazide labeling assay of fimbrillin suggested that it might be glycosylated.

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APA

Inoue, T., Ohta, H., Tanimoto, I., Shingaki, R., & Fukui, K. (2000). Heterogeneous post-translational modification of Actinobacillus actinomycetemcomitans fimbrillin. Microbiology and Immunology, 44(8), 715–718. https://doi.org/10.1111/j.1348-0421.2000.tb02554.x

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