Ketamine inhibits nitric oxide synthase in lipopolysaccharide-treated rat alveolar macrophages

52Citations
Citations of this article
23Readers
Mendeley users who have this article in their library.

Abstract

Purpose: To evaluate the effects of ketamine on the activity and protein expression of inducible nitric oxide synthase (iNOS) induced by lipopolysaccharide (LPS) in rat alveolar macrophages. Methods: Pulmonary alveolar macrophages isolated from Wistar-Kyoto rats were used. After incubation of macrophages with ketamine (1, 10, or 100 μM) and LPS (1 μg·ml-1)for 24 hr, the cell-free medium was removed for measuring the nitrite and tumour necrosis factor-a (TNF-α) levels by Griess reaction and ELISA kit, respectively, The harvested macrophages were also used to determine the activity of iNOS by using the conversion of [3H]-L-arginine to [3H]-L-citrulline method. In addition, the protein expression of iNOS was detected by Western blot analysis. Results: In rat alveolar macrophages, (i) ketamine (1 to 100 μM) caused a dose-dependent suppression of the production of nitrite and TNF-α induced by LPS and (ii) ketamine (1 00 μM) inhibited the activity (46.5 ± 4.8%, P < 0.05) and protein expression (35 ± 11%, P < 0.05) of iNOS in response to LPS. Conclusion: These results show that ketamine inhibits the activity and expression of iNOS in LPS-activated alveolar macrophages, which may be associated with the reduction of the release of TNF-α following LPS treatment.

Cite

CITATION STYLE

APA

Li, C. Y., Chou, T. C., Wong, C. S., Ho, S. T., Wu, C. C., Yen, M. H., & Ding, Y. A. (1997). Ketamine inhibits nitric oxide synthase in lipopolysaccharide-treated rat alveolar macrophages. Canadian Journal of Anaesthesia, 44(9), 989–995. https://doi.org/10.1007/BF03011971

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free