Native heart consists of myocytes and non-myocytes. We demonstrate here the feasibility of a size-based microfluidic separation of myocytes and non-myocytes from the neonatal rat myocardium. The device consists of a middle channel (50 μm wide, 200 μm tall, and 4 cm long) connected to adjacent side channels by microsieves (80 μm wide, 5 μm tall and 40 μm in length). The side channels increase in width in a flared shape along the length of the device to ensure constant pressure gradient across all sieves. In the first step, non-myoctes were removed from the myocytes by a conventional pre-plating method for 75 min. Subsequently, the non-myocytes were further enriched in a microfludic device at 20μ/min. We demonstrated that the cells in the middle and side channels maintained viability during sorting and the ability to attach and grow in culture. Upon culture for 48 h cardiomyocytes from the reservoir (control) and middle channel stained positive for cardiac Troponin I, exhibited a well developed contractile apparatus and contracted spontaneously and in response to electrical field stimulation. Most of the cells in the side channel expressed a non-myocyte marker vimetin. Fluorescent activated cell sorting indicated significant enrichment in the side channel (p < 0.001) for non-myocytes. Original cell suspension had a bimodal cell size distribution with the peaks in the range from 7-9 m and 15-17 μm. Upon cell sorting the distribution was Gaussian in both side channel and middle channel with the peaks in the range 7-9 μm and 9-11 μm respectively, indicating that the separation by size occurred. © Springer Science + Business Media, LLC 2006.
CITATION STYLE
Murthy, S. K., Sethu, P., Vunjak-Novakovic, G., Toner, M., & Radisic, M. (2006). Size-based microfluidic enrichment of neonatal rat cardiac cell populations. Biomedical Microdevices, 8(3), 231–237. https://doi.org/10.1007/s10544-006-8169-5
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