Additional pea EST-SSR markers for comparative mapping in pea (Pisum sativum L.)

Abstract

The availability of a large set of molecular markers is an excellent resource for both applied and basic research for any organism. The current consensus molecular map of pea is 1430cM and contains 239 simple sequence repeat markers. The goal was to identify and test unique gene-based simple sequence repeat markers to increase the available number for comparative mapping with sequenced legume species. A total of 6327 mRNA sequences were screened using a JAVA algorithm, culminating in the design of 96 novel, gene-based simple sequence repeat markers. The markers were tested for polymorphism between the parents of two recombinant inbred line mapping populations (Early Freezer 680×PI 269818 and C580257A×74SN3A) and 11 additional pea lines. A total of 45 new EST-SSR polymorphic markers were identified using these test lines, another 35 were monomorphic and the remaining 16 failed or produced substandard amplification profiles. Linkage map locations are presented for seven of the new EST-SSR markers. Primer sequence, repeat type, status within tested genotypes and the SSR-finding algorithm are presented. © 2011 Blackwell Verlag GmbH.