Immunofluorescence is a technique allowing the visualization of a specific protein or antigen in cells or tissue sections by binding a specific antibody chemically conjugated with a fluorescent dye such as fluorescein isothiocyanate. There are two major types of immunofluorescence staining methods: (1) direct immunofluorescence staining in which the primary antibody is labeled with fluorescence dye and (2) indirect immunofluorescence staining in which a secondary antibody labeled with fluorochrome is used to recognize a primary antibody. This chapter describes procedures for the application of indirect immunofluorescence staining to neural cells in culture. © 2012 Springer Science+Business Media, LLC.
CITATION STYLE
Barbierato, M., Argentini, C., & Skaper, S. D. (2012). Indirect immunofluorescence staining of cultured neural cells. Methods in Molecular Biology, 846, 235–246. https://doi.org/10.1007/978-1-61779-536-7_21
Mendeley helps you to discover research relevant for your work.