GlycoProtDB: A Database of Glycoproteins Mapped with Actual Glycosylation Sites Identified by Mass Spectrometry

Abstract

Asn(N)-linked glycans are attached to the consensus sequence ofN-glycosylation, Asn-Xaa-[Ser/Thr], where Xaa{\thinspace}≠{\thinspace}Pro, of a nascent protein by the oligosaccharyltransferase (OST) complex. Because the modification is carried out in the endoplasmic reticulum (ER), all predictable potential sequences are not necessarily glycosylated. Therefore, the information on the ``actual'' glycosylated site is important for glycobiology research. GlycoProtDB was constructed to provide such information collected by MS-based glycoproteomics technology. Now, the database contains the data of 3122 glycoproteins obtained from C. elegans, mouse, and human samples and is available at the URL: http://jcggdb.jp/rcmg/gpdb/index .