P-selectin is a multi-domain glycoprotein expressed on activated endothelial cells and activated platelets. We previously expressed a recombinant form of P-selectin containing only its N-terminal lectin and EGF domains in CHO-K1 cells and showed that these two domains are sufficient to mediate ligand binding. We have now expressed the same construct in CHO-Lec1 cells that make truncated glycans. The uniform glycosylation in these cells should make it easier to crystallize this protein.
CITATION STYLE
Mehta-D’souza, P. (2015). Immunodetection of P-selectin using an antibody to its C-terminal tag. In Western Blotting: Methods and Protocols (pp. 293–299). Springer New York. https://doi.org/10.1007/978-1-4939-2694-7_31
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