The neurosphere assay is a widely used method to culture neural precursor cells (NPCs), which include mixed populations of neural stem and progenitor cells, from the mammalian central nervous system. Fmr1-knockout (KO) mice generated to model fragile X syndrome (FXS) recapitulate the major phenotype of FXS. Neurosphere differentiation of cortical progenitors derived from brains of Fmr1-KO mice has been shown to reflect disordered mechanisms during cortical development in FXS in vivo. The cellular composition of neurospheres is heterogeneous, but robust FXS-specific alterations can be identified when culturing conditions are kept constant.
CITATION STYLE
Peteri, U. K., & Castrén, M. L. (2019). Modeling FXS with mouse neural progenitors. In Methods in Molecular Biology (Vol. 1942, pp. 71–78). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9080-1_6
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