Transposon tagging populations are important resources for characterizing gene function in model plant systems. In Setaria viridis, a transposon tagging population utilizing the miniature Ping (mPing) element was generated by introducing a construct containing mPing and the transposase Ping into accession A10.1. Transformants were screened for the presence of the transgene and mPing insertions were identified using nested inverse-PCR coupled with Illumina sequencing. Here we describe step-by-step protocols for generating the Setaria viridis mPing population, introduce methods for genotyping mPing insertion alleles, and discuss future implementations of the system.
CITATION STYLE
Kikuchi, K., Shyu, C., & Brutnell, T. P. (2017). Transposon Tagging in Setaria viridis (pp. 323–342). https://doi.org/10.1007/978-3-319-45105-3_19
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