Silencing FOXC1 inhibits growth and migration of human oral squamous cell carcinoma cells

Abstract

Forkhead box C1 (FOXC1) is a transcription factor that serves an important role in regulating tumorigenesis and cancer progression. However, the expression and functional role of FOXC1 in oral squamous cell carcinoma (OSCC) remains unclear. FOXC1 protein expression was determined using immunohistochemical staining of OSCC tissues and normal tissues. Cell Counting Kit-8, colony formation, migration and 5-ethynyl-2'-deoxyuridine assays were performed to investigate the role and underlying mechanism of action of FOXC1 in OSCC. A consistent increase in the immunoreactive intensity of FOXC1 in OSCC tissues as compared with that in adjacent normal tissues was demonstrated. Knockdown of FOXC1 impaired cell growth and colony formation by inhibiting cell proliferation and reducing cyclin B1 and cyclin D1 levels in OSCC cells. FOXC1-silenced OSCC cells exhibited decreased migration compared with that demonstrated by the control cells, accompanied by a downregulation of matrix metalloproteinase (MMP)-2 and MMP-9. Collectively, the results of the present study demonstrated that FOXC1 functions as an oncogene in OSCC and may be an important therapeutic target and predictive biomarker for OSCC.