Mating Behavior of Ascogaster reticulatus Watanabe (Hymenoptera: Braconidae), an Egg-Larval Parasitoid of the Smaller Tea Tortrix, Adoxophyes sp. (Lepidoptera: Tortricidae). III. Identification of a Sex Pheromone

Abstract

A sex pheromone of Ascogaster reticulatus Watanabe (Hymenoptera : Braconidae) which is deposited on the substrate by females and elicits male antennal-searching response, was analyzed and characterized. The hexane extract of the virgin females was subjected to silica gel column chromatography. An active fraction (active at 3.3×10-1_102 ng/line) was analyzed with gas chromatography (GLC) and GC-MS, and three major peaks were identified as citronellal (44%), hexadecanal (12%) and hexadecenal (43%). Double bond location at ninth position in hexadecenal was determined by GC-MS after dimethyl disulfide derivatization. Geometric isomers of 9-hexadecenal were both co-chromatographed on GLC and (Z)-9-hexadecenal was identical to the natural 9-hexadecenal. Bioassay with these authentic compounds showed that only (Z) -9-hexadecenal elicited pheromonal activity (active at 5×10-4_-5 ng/line). Therefore, (Z)-9-hexadecenal was identified as the sex pheromone of A. reticulatus. © 1991, JAPANESE SOCIETY OF APPLIED ENTOMOLOGY AND ZOOLOGY. All rights reserved.