Nonaqueous purification of maize mesophyll chloroplasts

Abstract

A nonaqueous fractionation method to obtain highly purifled mesophyll chloroplasts from lyophilized leaves of Zea mays L. is described. The levels of several metabolites including pyruvate were determined in the purified mesophyll chloroplast fractions which were prepared from leaves exposed to different light intensities. The role of pyruvate in the regulation of pyruvate, Pidikinase in these chloroplasts under different light intensities is discussed.