Mouse transgenesis in a single locus with independent regulation for multiple fluorophores

7Citations
Citations of this article
32Readers
Mendeley users who have this article in their library.

Abstract

A major barrier to complex experimental design in mouse genetics is the allele problem: combining three or more alleles is time-consuming and inefficient. Here, we solve this problem for transgenic animals with a simple modification of existing BAC transgenesis protocols, and generate triple-colored 'prism' mice in which the major cell types of the brain: neurons, astrocytes, and oligodendrocytes, are each labeled with a distinct fluorophore. All three fluorophores are expressed from the same locus, yet each fluorophore is expressed in an independent temporal and spatial pattern. All three transgenes are generally co-inherited across multiple generations with stable genomic copy number and expression patterns. This generic solution should permit more sophisticated experimental manipulations to assess functional interactions amongst populations of cell types in vivo in a more rapid and efficient manner. © 2012 Dougherty et al.

Cite

CITATION STYLE

APA

Dougherty, J. D., Zhang, J., Feng, H., Gong, S., & Heintz, N. (2012). Mouse transgenesis in a single locus with independent regulation for multiple fluorophores. PLoS ONE, 7(7). https://doi.org/10.1371/journal.pone.0040511

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free