PENGOMPOSAN SAMPAH ORGANIK DAN ISOLASI BAKTERI TERMOFIL DARI KOMPOS [COMPOSTING ORGANIC WASTE AND ISOLATING THERMOPHILE BACTERIA FROM COMPOST]

Abstract

Composting is one of the methods to sustainably minimize the accumulation of waste in the environment. Compost is made through the decomposition process of organic waste with the help of microorganisms. The process of making compost goes through several phases, one of the most important phases is the thermophilic phase. In this research, an evaluation was carried out on the effect of temperature, humidity, pH and aeration on the success of composting from leaves and twigs and braches of trembesi tree (Albizia saman). In addition, isolation and identification of thermophilic bacteria which play an important role in the composting process were carried out. Isolation of thermophilic bacteria is carried out when the compost making process is in the thermophilic phase. To support the growth of thermophilic bacteria, incubation during the isolation and purification process was carried out at a temperature of as high as 55-60 o C. Subsequently, thermophilic bacterial isolates were identified based on morphology and biochemical activity tests. The characterization results based on Bergey's Manual Systematic Bacteriology Second Edition Volume Three the Firmicutes indicated that the isolates obtained belonged to the genus Bacillus.