A universally applicable and rapid method for measuring the growth of Streptomyces and other filamentous microorganisms by methylene blue adsorption-desorption

Abstract

Quantitative assessment of growth of filamentous microorganisms, such as streptomycetes, is generally restricted to determination of dry weight. Here, we describe a straightforward methylene blue-based sorption assay to monitor microbial growth quantitatively, simply, and rapidly. The assay is equally applicable to unicellular and filamentous bacterial and eukaryotic microorganisms. © 2013, American Society for Microbiology.