Ribosome profiling is a powerful technique that enables researchers to monitor translational events across the transcriptome. It provides a snapshot of ribosome positions and density across the transcriptome at a sub-codon resolution. Here we describe the whole procedure of profiling ribosome footprints in mammalian cells. Two methods for Ribo-seq library construction are introduced, and their advantages and disadvantages are compared. There is a room for further improvement of Ribo-seq in terms of the amount of starting material, the duration of library construction, and the resolution of sequencing results.
CITATION STYLE
Qiu, J., & Qian, S. B. (2021). Poly-A Tailing and Adaptor Ligation Methods for Ribo-Seq Library Construction. In Methods in Molecular Biology (Vol. 2252, pp. 221–237). Humana Press Inc. https://doi.org/10.1007/978-1-0716-1150-0_10
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