Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida

  • Halimah H
  • Supramana S
  • et al.
Citations of this article
Mendeley users who have this article in their library.


Root-knot nematodes (RKN), Meloidogyne spp., were reported as the cause of carrot branched tuber on several vegetable production areas in Central and East Java. Species identification by molecular approach was conducted using infected carrot tubers from Agropolitan Cianjur, West Java. DNA was extracted from female nematodes and amplified using PCR with species specific primers (Fjav/Rjav for M. javanica, Far/Rar for M. arenaria, and Finc/Rinc for M. incognita) and multiplex primer (M. hapla, M. chitwoodi, and M. fallax). PCR product were sequenced without cloning. Based on nucleotide sequences, two species RKN were found associated with branched tuber disease of carrot in Agropolitan Cianjur, i.e M. javanica and M. hapla. Phylogenetic analysis showed that M. javanica from Cianjur was closely related to RKN from China with the homology level of 91.9%, whereas nucleotide sequence of M. hapla from Cianjur had high homology level (99.4% to 100%) with isolates from Swiss, USA, UK, and China.Key words: Meloidogyne hapla, M. javanica, phylogenetic, root-knot nematode




Halimah, H., Supramana, S., & Suastika, G. (2013). Identifikasi Spesies Meloidogyne pada Wortel berdasarkan Sikuen Nukleotida. Jurnal Fitopatologi Indonesia, 9(1), 1–6.

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free