Complex immunoprecipitation (Co-IP) is a powerful technique for precipitating an intact protein complex out of solution and cell lysates using an antibody that specifically binds to a particular protein in a large complex of proteins. Mass spectrometry (MS) is used to identify, sequence, and quantify proteins. RNA-induced silencing complexes (RISCs), Ago2 centered protein assemblies, are essential for miRNA mediated RNA decay and gene expression regulation; however, the complete list of RISCs is unknown. Here we describe methods used to combine IP and MS to identify new components of RISCs.
CITATION STYLE
Yi, T. (2018). Identifying RISC components using Ago2 immunoprecipitation and mass spectrometry. In Methods in Molecular Biology (Vol. 1720, pp. 149–159). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7540-2_11
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