Inhibition of hydrogen peroxide, nitric oxide and TNF-α production in peritoneal macrophages by ethyl acetate fraction from Alchornea glandulosa

Abstract

The effects of Alchornea glandulosa ethyl acetate fraction (AGF) on hydrogen peroxide (H2O2), nitric oxide (NO) and tumor necrosis factor-α (TNF-α) production in peritoneal macrophages activated with lipopolysaccharide (LPS) or phorbol myristate acetate (PMA) were investigated. Analysis by thin layer chromatography (TLC) of AGF showed several constituents, including flavonoids, which may have anti-inflammatory activity. Inhibitory effects of the fraction in H2O2 and NO production ranged from 8.59±7.84% to 70.56±4.16% and from 16.06±3.65% to 38.73±3.90%, respectively. The TNF-α production was only partially inhibited in the tested concentrations (12.21±6.23%-15.16±0.96%). According to these results, it is suggested that AGF has anti-inflammatory activity. This medicinal plant may have therapeutic potential in the control of inflammatory disorders. © 2005 Pharmaceutical Society of Japan.