Evaluation of the insecticidal efficacy of wild type and recombinant baculoviruses

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Abstract

A considerable amount of work has been undertaken to genetically enhance the efficacy of baculovirus insecticides. Following construction of a genetically altered baculovirus, laboratory bioassays are used to quantify various parameters of insecticidal activity such as the median lethal concentration (or dose) required to kill 50% of infected larvae (LC50 or LD50), median survival of larvae infected (ST 50), and feeding damage incurred by infected larvae. In this chapter, protocols are described for a variety of bioassays and the corresponding data analyses for assessment of the insecticidal activity of baculovirus insecticides.

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Popham, H. J. R., Ellersieck, M. R., Li, H., & Bonning, B. C. (2016). Evaluation of the insecticidal efficacy of wild type and recombinant baculoviruses. In Methods in Molecular Biology (Vol. 1350, pp. 407–444). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3043-2_21

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