Antifungal activity of metabolites of the endophytic fungus Trichoderma brevicompactum from garlic

Abstract

The endophytic fungus strain 0248, isolated from garlic, was identified asTrichoderma brevicompactum based on morphological characteristics and the nucleotide sequences of ITS1-5.8SITS2 and tef1. The bioactive compound T2 was isolated from the culture extracts of this fungus by bioactivity-guided fractionation and identified as 4β-acetoxy-12,13-epoxy-Δ9-trichothecene (trichodermin) by spectral analysis and mass spectrometry. Trichodermin has a marked inhibitory activity on Rhizoctonia solani, with an EC50 of 0.25 μgmL-1. Strong inhibition by trichodermin was also found for Botrytis cinerea, with an EC50 of 2.02 μgmL-1. However, a relatively poor inhibitory effect was observed for trichodermin againstColletotrichum lindemuthianum (EC50 = 25.60 μgmL-1). Compared with the positive control Carbendazim, trichodermin showed a strong antifungal activity on the above phytopathogens. There is little known about endophytes from garlic. This paper studied in detail the identification of endophytic T. brevicompactum from garlic and the characterization of its active metabolite trichodermin. © 2014, Sociedade Brasileira de Microbiologia.