Background: Vaccinia viruses have been used as a model for viral disease and as a protective live vaccine. Methodology and Principal Findings: We investigated the immunogenicity of an attenuated strain of vaccinia virus engineered to inactivate the N1L gene (vGK5). Using the intranasal route, this recombinant virus was 2 logs less virulent compared to the wild type VACV-WR. Infection by the intranasal, intraperitoneal, and tail scarification routes resulted in the robust induction of cytolytic virus-specific CD8 T cells in the spleens and the lungs. VACV-specific antibodies were also detected in the sera of mice infected 3-5 months prior with the attenuated vGK5 virus. Finally, mice immunized with vGK5 were significantly protected when challenged with a lethal dose of VACV-WR. Conclusions: These results indicate that the attenuated vGK5 virus protects against subsequent infection and suggest that the N1L protein limits the strength of the early antiviral CD8 T cell response following respiratory infection. © 2008 Mathew et al.
CITATION STYLE
Mathew, A., O’Bryan, J., Marshall, W., Kotwal, G. J., Terajima, M., Green, S., … Ennis, F. A. (2008). Robust intrapulmonary CD8 T cell responses and protection with an attenuated N1L deleted vaccinia virus. PLoS ONE, 3(10). https://doi.org/10.1371/journal.pone.0003323
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