Methyl groups are very useful probes of structure, dynamics, and interactions in protein NMR spectroscopy. In particular, methyl-directed experiments provide high sensitivity even in very large proteins, such as membrane proteins in a membrane-mimicking environment. In this chapter, we discuss the approach for labeling methyl groups in E. coli-based protein expression, as exemplified with the mitochondrial carrier GGC.
CITATION STYLE
Kurauskas, V., Schanda, P., & Sounier, R. (2017). Methyl-specific isotope labeling strategies for NMR studies of membrane proteins. In Methods in Molecular Biology (Vol. 1635, pp. 109–123). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7151-0_6
Mendeley helps you to discover research relevant for your work.