Newer fluorometric methods for the analysis of biologically important compounds

Abstract

Newer fluorometric methods for the analysis of biologically important compounds will be discussed: enzymes such as LDH, alkaline phosphatase, lipase and cholinesterase, and substrates such as glucose, urea and uric acid. These methods are based on the production of fluorescence initiated by an enzymic reaction. New reagentless fluorescence methods will be described for enzymes and substrates. These methods are highly precise (1%), fast (less than 1 minute) and involve no preparation of reagents. These methods, as adapted to clinical laboratory procedures, will be discussed.