An effective method to quantify the parasite loads is the key to the evaluation of anti-cryptosporidial drug efficacy in vitro. However, high-throughput screening (HTS) of drugs against Cryptosporidium parvum in vitro was impractical by the labor-intensive traditional assays. Here we describe a simplified quantitative RT-PCR assay suitable for HTS of compounds and for evaluating drug efficacy against the growth of C. parvum in vitro.
CITATION STYLE
Zhang, H., & Zhu, G. (2020). High-Throughput Screening of Drugs Against the Growth of Cryptosporidium parvum In Vitro by qRT-PCR. In Methods in Molecular Biology (Vol. 2052, pp. 319–334). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9748-0_18
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