Direct-injection mass spectrometry (DIMS) is a means of rapidly obtaining metabolomic phenotype data in both prokaryotes and eukaryotes. Given our generally poor understanding of Campylobacter metabolism, the high-throughput and relatively simple sample preparation of DIMS has made this an attractive technique for metabolism-related studies and hypothesis generation, especially when attempting to analyze metabolic mutants with no clear phenotype. Here we describe a metabolomic fingerprinting approach with sampling and extraction methodologies optimized for direct-injection electrospray ionization mass spectrometry (ESI-MS), which we have used as a means of comparing wild-type and isogenic mutant strains of C. jejuni with various metabolic blocks.
CITATION STYLE
Howlett, R. M., Davey, M. P., & Kelly, D. J. (2017). Metabolomic analysis of Campylobacter jejuni by direct-injection electrospray ionization mass spectrometry. In Methods in Molecular Biology (Vol. 1512, pp. 189–197). Humana Press Inc. https://doi.org/10.1007/978-1-4939-6536-6_16
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