Characteristics of donor-specific antibodies associated with antibody-mediated rejection in lung transplantation

Abstract

Although donor-specific anti-human leukocyte antigen (HLA) antibodies (DSAs) are frequently found in recipients after lung transplantation (LT), the characteristics of DSA which influence antibody-mediated rejection (AMR) in LT are not fully defined. We retrospectively analyzed 206 consecutive LT patients of our center (2010-2013). DSAs were detected by using luminex single antigen beads assay and mean fluorescence intensity was assessed. Within the study population, 105 patients had positive DSA. Patients with and without AMR (AMR Pos , n = 22, and AMR Neg , n = 83, respectively) were compared. AMR Pos patients had significantly greater frequencies of anti-HLA DQ DSA (DQ DSA) than AMR Neg patients (95 vs 58%, respectively, p < 0.0001). Compared to AMR Neg patients, AMR Pos patients had higher DQ DSA sum MFI [7,332 (2,067-10,213) vs 681 (0-1,887), p < 0.0001]. DQ DSA when associated with AMR, had more frequent graft loss and chronic lung allograft dysfunction (CLAD). These data suggest (i) that DSA characteristics clearly differ between AMR Pos and AMR Neg patients and (ii) the deleterious impact of DQ DSA on clinical outcome.