Investigating metabolic changes during different organismal or cellular states is of increasing interest. The combination of a data-rich analytical method like mass spectrometry or NMR spectroscopy with a statistical analysis identifies metabolites that are affected by a certain stimulus. Thus, important information on the underlying molecular pathways can be obtained. Here, we describe how to investigate metabolic changes in a model of oncogene-induced senescence. The water-soluble metabolites are isolated by a chloroform-methanol treatment and subsequently analyzed by NMR spectroscopy.
CITATION STYLE
Gey, C., & Seeger, K. (2017). Metabolic changes investigated by proton NMR spectroscopy in cells undergoing oncogene-induced senescence. Methods in Molecular Biology, 1534, 155–163. https://doi.org/10.1007/978-1-4939-6670-7_15
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