A simple method for site-directed mutagenesis using the polymerase chain reaction

Abstract

Incorrect details regarding PCR conditions were supplied for the Materials and Methods section of this paper. The correct details are published below.PCR conditions. Template DNA (10 fmol) and primer sets (1 μM each) were incubated in a Perkin Elmer Cetus Thermal Cycler in 100 μl reaction volumes containing 10 mM Tris-HCl pH 8.3, 50 mM KCl, 2.5 mM MgCl2, 10 μg gelatin, 0.2 mM of each of NTP and 2 units Taq polymerase (5). © 1989 IRL Press.