Development of an accurate and cost-effective quantitative detection method for specific gene sequences - Development of a quantitative detection method for specific gene sequences using fluorescence quenching phenomenon

Abstract

DNA and RNA quantifications are essential in various fields such as biomedicine, agriculture, fishery, environment, and food. We have developed an accurate and cost-effective method for the quantification of specific nucleic acid sequences; the method involves the use of the fluorescent quenching phenomenon via an electron transfer between the dye and a guanine base at a particular position. This paper describes the elemental key technologies and their synthesis for the development of such a gene quantification method. Furthermore, based on the findings of a collaborative research project with a private company, we report the scenario for the industrialization and the practical use of the developed method.