Vitamin B 12 uptake across the mycobacterial outer membrane is influenced by membrane permeability in Mycobacterium marinum

Abstract

Our study investigates how mycobacteria acquire essential vitamin B 12 . These microbes, including those causing tuberculosis, face challenges in nutrient uptake due to their strong outer layer. We focused on Mycobacterium marinum , similar to TB bacteria, to uncover its vitamin B 12 absorption. We used modified strains unable to produce their own B 12 and discovered that M. marinum can indeed absorb it from the environment, even during infections. Changes in the outer layer composition affect this process, and genes related to membrane integrity play key roles. These findings illuminate the interaction between mycobacteria and their environment, offering insights into combatting diseases like tuberculosis through innovative strategies. Our concise research underscores the pivotal role of vitamin B 12 in microbial survival and its potential applications in disease control.