Purpose: To evaluate the effects of alcohol and caffeine in a pancreatic carcinogenesis mouse model induced by 7,12-dimethylbenzantracene (DMBA), according to the PanIN classification system. Methods: 120 male, Mus musculus, CF-1 mice were divided into four groups. Animals received either water or caffeine or alcohol or alcohol + caffeine in their drinking water. In all animals, 1 mg of DMBA was implanted into the head of the pancreas. After 30 days, euthanasia was performed; excised pancreata were then fixed in formalin, stained with hematoxylin-eosin and categorized as follows: normal ducts, reactive hyperplasia, PanIN-1A, PanIN-1B, PanIN-2, PanIN-3 or adenocarcinoma. Results: PanIN lesions were verified in all groups. Adenocarcinoma was detected in 15% of animals in the caffeine group, 16.6% in the water group, 23.8% in the alcohol + caffeine group and 52.9% in the alcohol group (P<0.05). Conclusions: The experimental pancreatic carcinogenesis mouse model using DMBA effectively induces PanIN lesions and pancreatic adenocarcinoma. This study verified the association between alcohol use and pancreatic adenocarcinoma; caffeine did not present the same effect.
CITATION STYLE
Wendt, L. R. R., Osvaldt, A. B., Bersch, V. P., Schumacher, R. D. C., Edelweiss, M. I. A., & Rohde, L. (2007). Pancreatic intraepithelial neoplasia and ductal adenocarcinoma induced by DMBA in mice. Effects of alcohol and caffeine. Acta Cirurgica Brasileira, 22(3), 202–209. https://doi.org/10.1590/S0102-86502007000300008
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