Functional assays for hematopoietic stem cell self-renewal.

Abstract

Stem cells are defined by the ability to self-renew. Specific functional assays have been developed for the rigorous identification and quantification of hematopoietic stem cells (HSCs), making these cells the benchmark in studies of self-renewal. Here, we review the theory behind these functional stem cell tests and discuss important considerations in choosing and designing these assays. Finally, we provide a basic protocol for the serial-dilution assay, a quantitative assay for HSCs, from which individual researchers can construct their own customized protocols utilizing the guidelines discussed.